目的：研究在上海地区健康汉族人中不同年龄和性别的载脂蛋白Ｅ基因频率的群体分布．方法：本次调查包括６４９名健康汉族人（女：２３７人，男：４１２人）．载脂蛋白Ｅ基因用ＰＣＲ扩增，前引物：５′ＧＧＣＡＣＧＧＣＴＧＴＣＣＡＡＧＧＡＧＣＴ３′；后引物：５′ＧＡＴＧＧＣＧＣＴＧＡＧＧＣＣＧＣＧＣＴ３′．ＰＣＲ产物用５单位限制性内切酶ＣｆｏⅠ酶切，用２０％聚丙烯酰胺凝胶电泳分离．结果：ＡｐｏＥ３频率最高为８６４％，其次ＡｐｏＥ４频率为７５％，ＡｐｏＥ２频率最低为６２％．经χ２检验，等位基因及基因型频率分布符合ＨａｒｄｙＷｅｉｎｂｅｒｇ平衡．结论：本项研究提供了正常中国汉族人群中载脂蛋白Ｅ三个等位基因２，３和４频率的数据 OBJECTIVE: To study the population distribution of apolipoprotein E gene frequencies at different ages and genders among healthy Han Chinese in Shanghai. Methods: The survey included 649 healthy Han Chinese (237 females and 412 males). Apolipoprotein E gene amplified by PCR, the first primer: 5’GGCACGGCTGTCCAAGGAGCT 3 ’; after primer: 5’GATGGCGCTGAGGCCGCGCT 3’. The PCR product was digested with 5 units of restriction enzyme Cfo I and separated by 20% polyacrylamide gel electrophoresis. Results: The highest ApoE3 frequency was 86.4%, followed by ApoE4 at 75% and ApoE2 at 62%. By χ2 test, allele and genotype frequency distribution in line with the Hardy  Weinberg equilibrium. CONCLUSIONS: This study provides data on the frequencies of the three alleles 2, 3, and 4 of apolipoprotein E in normal Chinese Han population