论文部分内容阅读
目的建立安胃片中有效成分原阿片碱和延胡索乙素的测定法。方法高效液相色谱法(HPLC)法同时测定安胃片中原阿片碱和延胡素乙素的含量;色谱柱Agilent Eclipse-C18(250 mm×4.6 mm,5μm);流动相乙腈-0.6%醋酸溶液(用三乙胺调节p H6.0);梯度洗脱,流速1.0 ml/min;检测波长280 nm;柱温26℃。结果该方法中原阿片碱在进样量0.0560~2.2400μg内呈良好的线性关系(r=0.9997);延胡索乙素在进样量0.0190~0.7613μg内呈良好的线性关系(r=0.9998)。结论以上方法方便可靠,可作为安胃片的质控方法。
Objective To establish an assay for the determination of protopine and tetrahydropalmatine in Anwei tablets. Methods The contents of protopine and fevanin in Anwei tablets were determined by high performance liquid chromatography (HPLC). The mobile phases were Agilent Eclipse-C18 (250 mm × 4.6 mm, 5 μm), mobile phase acetonitrile-0.6% acetic acid solution Adjust pH6.0 with triethylamine; gradient elution at a flow rate of 1.0 ml / min; detection wavelength at 280 nm; Results The method showed good linearity (r = 0.9997) for protopine in the injection volume of 0.0560 ~ 2.2400μg. Tetrahydropalmatine showed a good linearity (r = 0.9998) in the range of 0.0190 ~ 0.7613μg. Conclusion The above method is convenient and reliable, which can be used as quality control method of Anwei tablets.