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目的探讨白细胞介素6、8（IL－6、IL－8）以及肿瘤坏死因子（TNF－α）在病毒性脑炎中的作用。方法采用ELISA法检测35例病毒性脑炎和28例健康儿童血清及外周上单个核细胞（PBMC）请生的IL-6、IL-8、TNF－α水平，以及外用血T细胞及亚群。结果病脑患儿血清IL－8、TNF－α水平显著高于对照组，且随病情加重呈增高趋势；体外PBMC诱生IL-6、IL-8、TNF－α水平均明显高于对照组；重型病人TNF－α水平高于轻型病人；病脑组CD3+、CD4+、CD8+细胞百分率及淋巴细胞转化率均较对照组明显降低。结论小儿病脑存在多种细胞因子失调，其中IL－8、TNF－α在其炎症反应及脑损伤过程中可能起重要作用。
Objective To investigate the role of IL-6, IL-8 and tumor necrosis factor (TNF-α) in viral encephalitis. Methods The levels of IL-6, IL-8 and TNF-α in peripheral blood and peripheral blood mononuclear cells (PBMCs) from 35 viral encephalitis and 28 healthy children were measured by ELISA. T cell subsets . Results The serum levels of IL-8 and TNF-α in the sick children were significantly higher than those in the control group, and increased with the severity of illness. The levels of IL-6, IL-8 and TNF-α induced by PBMC in vitro were significantly higher than those in the control group . The level of TNF-α in severe patients was higher than that in the light patients. The percentage of CD3 +, CD4 +, CD8 + cells and lymphocyte transformation rate in the diseased brain group were significantly lower than those in the control group. Conclusion There are many cytokine imbalances in pediatric patients. IL-8 and TNF-α may play an important role in the inflammatory reaction and brain injury.