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作者曾构建了质粒载体pSG5rab.gp,该质粒含有猿猴病毒40(SV40)启动子控制下的狂犬病病毒G蛋白(RVGP)的全长cD-NA,肌肉注射小鼠后可诱生特异性B细胞和T细胞介导的免疫应答,并能保护小鼠抵抗毒力株攻击。本研究中作者测试了可能影响该DNA疫苗效力的两个因素:(1)不同的启动子;(2)分泌型与膜结合型RVGP。
The authors have constructed the plasmid vector pSG5rab.gp which contains the full-length cD-NA of rabies virus G protein (RVGP) under the control of the simian virus 40 (SV40) promoter and induces specific B cells after intramuscular injection And T cell-mediated immune responses and protect mice against virulent challenge. The authors tested two factors that could affect the efficacy of the DNA vaccine: (1) different promoters; and (2) secreted and membrane-bound RVGP.