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目的探讨ｐ１６基因缺失和突变与人非小细胞肺癌发生、发展的关系。方法应用多重ＰＣＲ、ＳＳＣＰ分析及ＤＮＡ序列分析技术，对４５例人非小细胞肺癌组织中ｐ１６基因外显子１和外显子２进行基因缺失和突变分析。结果４５例肺癌样品中共检出１９例ｐ１６基因缺失，总的缺失率为４２．２％（１９／４５）。其中纯合型缺失８例（１７．８％），半合型缺失１１例（２４．４％）；外显子１缺失７例（１５．６％），外显子２缺失１２例（２６．７％）；ｐ１６基因外显子２的突变检出率为３１．１％（１４／４５）；ＤＮＡ序列分析显示ｐ１６基因７２位密码子无义突变７例，７５位密码子错义突变７例。结论ｐ１６基因的缺失和突变可能与人非小细胞肺癌的发生、发展有关。
Objective To investigate the relationship between the deletion and mutation of p16 gene and the occurrence and development of human non-small cell lung cancer. Methods The gene deletion and mutation analysis of exon 1 and exon 2 of p16 gene in 45 human non-small cell lung cancer tissues were performed by multiplex PCR, SSCP analysis and DNA sequence analysis. Results A total of 19 p16 gene deletions were detected in 45 lung cancer samples. The total deletion rate was 42.2% (19/45). Among them, 8 were homozygous (17.8%), 11 were hemizygous (24.4%), 7 were exon 1 (15.6%), and 12 were exon 2 (26 The mutation detection rate of exon 2 of p16 gene was 31.1% (14/45). DNA sequence analysis showed that there were 7 non-sense mutations in codon 72 of p16 gene and 75 codon missense mutations. 7 cases. Conclusion The deletion and mutation of p16 gene may be related to the occurrence and development of human non-small cell lung cancer.