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目的建立测定复方思日颗粒中绿原酸含量的方法。方法采用Waters Sun Fire TM C18(150mm×4.6mm,5μm)色谱柱,流动相为甲醇(A)-乙腈(B)-0.4%磷酸水溶液(C),梯度洗脱(0~50min,5.5%A→6%A,2.9%B,91.6%C→91.1%C),柱温30℃,流速1.0mL/min,检测波长327nm,进样量15μL。结果绿原酸对照品在4.24~25.44μg/mL浓度范围内与峰面积的线性关系良好。加标回收率为98.739%(n=9),RSD为2.622%,分离度>1.5,对称因子不得低于0.7。结论 HPLC方法简便、结果准确,可用于复方思日颗粒中绿原酸的含量测定。
Objective To establish a method for determination of chlorogenic acid in Fufang Siri particles. Methods A Waters Sun Fire C18 (150mm × 4.6mm, 5μm) column was used. The mobile phase consisted of methanol (A) -acetonitrile (B) -0.4% phosphoric acid (C) → 6% A, 2.9% B, 91.6% C → 91.1% C). The column temperature was 30 ℃, the flow rate was 1.0 mL / min, the detection wavelength was 327 nm and the injection volume was 15 μL. Results The calibration curve of chlorogenic acid in the range of 4.24 ~ 25.44μg / mL had a good linear relationship with the peak area. The spiked recoveries were 98.739% (n = 9), RSD was 2.622%, resolution was> 1.5 and symmetry factor was not lower than 0.7. Conclusion The HPLC method is simple and accurate. It can be used for the determination of chlorogenic acid in Compound Siri Granule.