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目的利用人胃癌细胞系(GC9811)在裸小鼠体内反复接种建立一株具有腹膜高转移潜能的胃癌细胞系(GC9811P),并对其生物学特性进行观察,为实验研究提供模型。方法采用胃癌细胞系(GC9811)在裸小鼠腹腔内反复接种,行体外培养腹膜转移灶筛选高转移亚系,绘制细胞生长曲线,光镜、电镜下观察细胞形态,利用流式细胞仪、染色体分析等方法,研究该高转移亚系的细胞周期、增殖、染色体核型等生物学特性。结果母本细胞系GC9811腹膜转移形成率为33.3%(3/10),而GC9811P腹膜转移形成率为100%。两种细胞系腹膜结节组织学形态大体相似。增殖速度较母系加快。细胞周期分析G1期53.5%、G2期12.5%、S期37.1%。且遗传学特性包括染色体形态仍为人类核型,众数维持在104～126之间,占70%。结论具有腹膜高转移的胃癌细胞系GC9811P的建立及裸小鼠体内实验模型,为研究胃癌腹膜转移机制及探索新的治疗途径提供了极为有用的工具。
Objective To establish a gastric cancer cell line (GC9811P) with high peritoneal metastasis potential by repeated inoculation of human gastric cancer cell line (GC9811) in nude mice and observe its biological characteristics to provide a model for experimental research. Methods The gastric cancer cell line (GC9811) was used to inoculate nude mice intraperitoneally repeatedly. The peritoneal metastases were cultured in vitro to screen the hyper-metastatic subtypes. The cell growth curve was drawn. The cell morphology was observed under light microscope and electron microscope. Analysis and other methods to study the biological characteristics of the highly metastatic sub-line cell cycle, proliferation, chromosome karyotype. Results The rate of peritoneal metastasis of GC9811 was 33.3% (3/10), while the rate of peritoneal metastasis of GC9811P was 100%. The histological morphology of peritoneal nodules was similar in both cell lines. Proliferation faster than maternal. Cell cycle analysis showed G1 phase 53.5%, G2 phase 12.5% and S phase 37.1%. And genetic characteristics, including chromosome morphology is still human karyotype, the majority remained at 104 to 126, accounting for 70%. Conclusion The establishment of gastric cancer cell line GC9811P with peritoneal hypermetastasis and in vivo experimental model in nude mice provide an extremely useful tool for studying the mechanism of peritoneal metastasis of gastric cancer and exploring new therapeutic approaches.