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目的观察小鼠体内传代与氟康唑体外诱导对阿萨希毛孢子菌(T.asahii)形态及药物敏感性的影响。方法将4株T.asahii在小鼠体内5次传代后,观察传代前后菌株形态和氟康唑最小抑菌浓度(minimal inhibitory concentration,MIC);选择临床株CBS 2479和环境株CBS 8904,在含不同浓度氟康唑的固体培养基中体外诱导,观察诱导前后菌株形态和MIC值的变化。结果体内传代后所有菌株的MIC值和菌落形态较传代前均有较明显变化,其中临床株的MIC值较传代前增长8倍,而环境株的光镜下形态变化较明显,从以孢子状态为主变为孢子和菌丝的混合状态。体外诱导后,临床株和环境株的MIC值均>256μg/ml,以临床株的MIC值变化幅度最大,其光镜下细胞形态也从诱导前的菌丝状态为主转化为孢子状态为主。结论 T.asahii体内传代及体外诱导前后形态及药物敏感性均发生变化。体内传代后环境株形态有向临床株转变的趋势,而体外诱导后临床株逐渐向环境株转变。临床株标准株两种情况下氟康唑MIC值增高均以临床株更为显著,体外诱导较体内传代氟康唑MIC值增长更高、更快。
Objective To observe the effects of in vitro passage and fluconazole induction on the morphology and drug sensitivity of T.asahii in mice. Methods Four strains of T.asahii were passaged in vivo and the morphology and fluconazole minimum inhibitory concentration (MIC) of the four T.asahii strains were observed before and after passage. The clinical strains CBS 2479 and CBS 8904 were selected. Different concentrations of fluconazole solid medium induction in vitro, observed before and after induction of strain morphology and MIC value changes. Results The MIC values and colony morphology of all the strains after passage were significantly changed compared with those before passage. The MIC value of clinical strains increased by 8 times compared with that before passage, while the morphological changes of the strains were obvious under the light microscope. Mainly into a mixture of spores and mycelium. After in vitro induction, MIC values of clinical strains and environmental strains were both> 256μg / ml, and the MIC value of clinical strains had the largest change. Under light microscopy, the morphology of mycelium was predominantly transformed to spore state . Conclusion The morphological and drug sensitivity changes of T.asahii in vitro before and after passage and induction were observed. After passage, the morphological changes of the clinical isolates from the clinical isolates were observed. However, the in vitro clinical isolates gradually transformed into the environmental isolates. Clinical strains of standard strains in both cases, fluconazole MIC values were more significant clinical strains, in vitro induction of flu than the body pass MIC values ?? to grow higher and faster.