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目的探讨不同脂肪酸对胰岛β细胞功能及内质网应激(ERS)的影响。方法选取40只大鼠分为正常饮食对照(N)组、单不饱和脂肪酸饮食(M)组、多不饱和脂肪酸饮食(P)组和饱和脂肪酸饮食(S)组。干预8周行静脉葡萄糖耐量实验(IVGTT),测定胰腺磷酸化真核生物翻译起始因子2的α亚基(p-eIF2α)和CCAAT/增强子结合蛋白(C/EBP)同源蛋白(CHOP)的表达。结果8周末,S组IVGTT胰岛素第1、3、5、8min均较N组降低(P<0.05)。M、P、S组急性胰岛素反应(AIR)均较N组降低(P<0.05或P<0.01)。P、S组胰腺β细胞内p-eIF2α和CHOP的表达较N组增加(P<0.05或P<0.01)。结论不同高脂肪酸饮食均可造成胰岛β细胞功能损伤,可能与ERS有关。
Objective To investigate the effect of different fatty acids on pancreatic β-cell function and endoplasmic reticulum stress (ERS). Methods Forty rats were divided into normal diet control (N) group, monounsaturated fatty acid diet (M) group, polyunsaturated fatty acid diet (P) group and saturated fatty acid diet (S) group. Intervenient venous glucose tolerance test (IVGTT) was performed for 8 weeks. The levels of α-subunit (p-eIF2α) and CCAAT / enhancer binding protein (C / EBP) homologue of pancreatic phosphorylation eukaryotic translation initiation factor 2 )expression. Results At the end of 8th week, the IVGTT insulin in group S was lower than that in group N at 1, 3, 5 and 8 min (P <0.05). The acute insulin response (AIR) in M, P and S groups was lower than that in N group (P <0.05 or P <0.01). The expression of p-eIF2α and CHOP in pancreatic β-cells of P and S groups increased compared with N group (P <0.05 or P <0.01). Conclusion Different high-fat diet can cause pancreatic β-cell dysfunction, which may be related to ERS.