论文部分内容阅读
目的检测IL-18、VEGF在正常角膜和血管化角膜的表达,探讨IL-18在角膜新生血管化中的作用。方法收集角膜移植手术后病变角膜23例,正常角膜10例,采用免疫组织化学染色方法检测IL-18、VEGF在正常角膜和血管化角膜各层组织的表达。结果正常角膜组织中IL-18主要分布在上皮层、前弹力层、内皮层,以上皮层的表达最明显,VEGF在上皮层、前弹力层、和内皮层均有表达,前弹力层表达最强烈;血管化的角膜标本中IL-18表达相对正常角膜降低(P<0.001),而VEGF在该标本中上皮层、基质层均强烈表达,新生血管化区域、炎性细胞均呈现强阳性,相对正常角膜明显增强(P<0.001);二者表达呈负相关(γ=-0.683,P<0.05)。结论血管化角膜中IL-18水平明显降低,角膜新生血管化病理过程中新生血管的形成与IL-18水平降低密切相关。
Objective To detect the expression of IL-18 and VEGF in normal cornea and vascularized cornea, and to explore the role of IL-18 in corneal neovascularization. Methods Twenty-three patients with corneal lesions and 10 normal corneas were collected after corneal transplantation. Immunohistochemical staining was used to detect the expression of IL-18 and VEGF in normal cornea and vascularized cornea. Results The expression of IL-18 in epithelial, anterior elastic layer, endothelium and cortex was the highest in normal corneal epithelium. The expression of VEGF in epithelium, anterior elastic layer and endothelium was the highest ; The expression of IL-18 in vascularized corneal samples was lower than that in normal cornea (P <0.001), while VEGF was strongly expressed in the epithelium and stroma of the specimens, while the neovascularization area and inflammatory cells showed strong positive The normal cornea was significantly enhanced (P <0.001), and the expression was negatively correlated (γ = -0.683, P <0.05). Conclusions The level of IL-18 in vascularized corneas is significantly decreased. The formation of neovascularization during corneal neovascularization is closely related to the decrease of IL-18 level.