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枸杞多糖(lycium barbarum polysaccharide,LBP)是从传统中药枸杞子中提取的有效成分,免疫增强作用是其主要药理活性,本文进一步研究了枸杞糖2(LBP2)对SD大鼠腹腔巨噬细胞(MΦ)功能的影响.1 材料与方法1.1 试剂与药品 脂多糖(LPS)、中性红均购自Sigma公司,放线菌素D为Fluka产品,rhTNF购自中国军事医学科学院四环公司,结晶紫购自上海崇明化学试剂厂.枸杞多糖2(LBP2)为本室所提取,溶于RPMI-1640培养液后过滤除菌,分装冻存备用.
Lycium barbarum polysaccharide (LBP) is an effective component extracted from the traditional Chinese medicine gardenia. The immune enhancement is its main pharmacological activity. This article further studied the effect of LBP2 on peritoneal macrophages of SD rats (MΦ ) The effects of the function. 1 Materials and methods 1.1 Reagents and pharmaceuticals LPS, neutral red were purchased from Sigma, Actinomycin D was Fluka, rhTNF was purchased from China National Academy of Military Medical Sciences Fourth Ring, Crystal Violet Purchasing from Shanghai Chongming Chemical Reagent Factory. Lycium barley polysaccharide 2 (LBP2) is extracted from this room and dissolved in RPMI-1640 medium. It is filtered, sterilized, and frozen for storage.