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鼓膜穿刺可确定中耳炎的病原菌,但是创伤性的 并伴有疼痛,故需要一种快速无创的技术进行细菌学诊断。应用荧光发射分光光度法对四种常见病原菌和真灰鼠鼓膜进行研究,建立了中耳炎的荧光细菌学诊断标准并讨论了有关技术和设计。以嗜血性流感杆菌B、摩拉克氏卡他菌、金葡菌和肺炎球菌为研究对象。取细菌在每升含有氯化钠10g、胰蛋白胨10g、酵母5g的肉汤中增殖,温度控制在37℃,离心后在无菌盐水中洗涤,最后用600nm波长测量1cm的细菌浓度是0.1光密度。用ISS Greg200分光光度计测量荧光光谱。刺激
Tympanostomy can identify otitis media pathogens, but traumatic and accompanied by pain, so the need for a rapid non-invasive technology for bacteriological diagnosis. Fluorescence spectrophotometry was used to study four kinds of common pathogenic bacteria and true squirrel eardrum, and the diagnostic criteria of fluorescent bacteriology of otitis media were established. The related technologies and designs were discussed. Haemophilus influenzae B, Moraxella catarrhalis, Staphylococcus aureus and pneumococcus were studied. Bacteria were inoculated in broth containing 10 g of sodium chloride, 10 g of tryptone and 5 g of yeast at a temperature controlled at 37 ° C. After centrifugation, the cells were washed in sterile saline. Finally, the bacterial concentration of 1 cm measured at a wavelength of 600 nm was 0.1 light density. Fluorescence spectra were measured using an ISS Greg200 spectrophotometer. stimulate