据悉,近日国家知识产权局公开一件由中国农业科学院烟草研究所申请的发明专利:一种根结线虫分子鉴定引物集及其制备方法与应用。该发明提供了一种根结线虫分子鉴定引物集,通过如下方法获得:利用预测的南方根结线虫蛋白序列分别与南方根结线虫基因组和北方根结线虫基因组比对,设置比对序列的相似度即percent参数为75,得到单拷贝基因;将单拷贝基 It is reported that the State Intellectual Property Office has recently opened an application by the Chinese Academy of Agricultural Sciences Institute of Tobacco patents for invention: a root-knot nematode molecular identification primer set and its preparation methods and applications. The invention provides a root-knot nematode molecular identification primer set, which is obtained through the following methods: comparing predicted root-knot nematode protein sequences with the genomes of the root-knot nematode genome and the root-knot nematode genome respectively, and setting the similarity of the aligned sequences Degrees that the percent parameter is 75, get a single copy of the gene; the single copy base