论文部分内容阅读
为研究巨细胞病毒（ＣＭＶ）感染免疫细胞能力，本文选择６株从婴儿肝炎综合征患儿尿中新分离的ＣＭＶ体外感染健康人外周血各类由细胞后检测ＣＭＶ早期抗原，结果显示新分离病毒体外可感染粒细胞、单核细胞、淋巴细胞表达早期抗原，其感染阳性率明显高于已适应实验室生长的ＣＭＶＡＤ１６９株病毒，培养液中加入ｒＩＬ２、ｒＩＬ２、ＩＦＮ－γ可增加分离病毒对淋巴细胞的感染，而加入丝裂原ＰＨＡ、ＣｏｎＡ无此作用。提示巨细胞病毒感染免疫细胞能力较强。
In order to study the ability of cytomegalovirus (CMV) to infect immune cells, six CMV newly isolated from the urine of infants with hepatitis syndrome were selected to detect CMV early antigens in peripheral blood of healthy people in vitro. The results showed that the newly isolated The virus can infect granulocytes, monocytes and lymphocytes to express early antigens in vitro. The positive rate of infection is obviously higher than that of CMVAD169 strain that has been adapted to laboratory growth. Adding rIL2, rIL2 and IFN-γ to the culture solution can increase the isolation of virus Lymphocytic infection, while the addition of mitogen PHA, ConA no such effect. Tip cytomegalovirus infection of immune cells ability.