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目的探讨自聚肽纳米纤维支架承载骨髓源性心肌干细胞(MCSC)移植对大鼠心肌梗死后心功能恢复的影响,寻找一种更适合承载心肌干细胞治疗心肌梗死的可注射性生物材料。方法通过单细胞克隆培养技术,从骨髓间充质干细胞中筛选具有心肌特异分化潜能的MCSC,固相合成自聚多肽。将雌性大鼠心肌梗死模型随机分为3组:对照组、MCSC移植组、支架承载MCSC移植组。细胞移植后4周,用M型超声心动图检测心功能恢复情况;对心脏组织切片进行Masson染色,利用图像分析检测胶原纤维比率;用原位荧光杂交法标记心肌组织中Y染色体细胞,并检测其心肌肌钙蛋白T(cTnT)的表达。结果支架承载MCSC移植组与MCSC移植组相比,大鼠的心功能指数明显提高,胶原纤维比率明显减小,Y染色体阳性细胞数明显增多,差异均具有统计学意义。结论支架承载MCSC移植治疗心肌梗死,有利于移植细胞在受损心肌内的存活和向功能性心肌分化,并促进心功能恢复,其疗效优于单纯干细胞移植。
OBJECTIVE: To investigate the effect of autologous poly-peptide nanofibrous scaffold bearing bone marrow-derived cardiac stem cells (MCSC) on the recovery of cardiac function after myocardial infarction in rats and to find out an injectable biomaterial that is more suitable for carrying cardiac stem cells for myocardial infarction. Methods MCSCs with myogenic differentiation potential were isolated from bone marrow mesenchymal stem cells by single cell cloning technique. The female rats were randomly divided into 3 groups: control group, MCSC transplantation group and MCSC transplantation group. Four weeks after cell transplantation, heart function recovery was detected by M-mode echocardiography. Masson staining of heart tissue sections was performed to detect collagen fiber ratio by image analysis. Y chromosome cells in myocardium were labeled by in situ fluorescent hybridization and detected Its cardiac troponin T (cTnT) expression. Results Compared with MCSC group, the cardiac function index of MCSC group was significantly increased, the ratio of collagen fibers was significantly decreased and the number of Y chromosome positive cells was significantly increased in MCSC group. The differences were statistically significant. Conclusion MCSC transplantation for myocardial infarction is beneficial to the survival and functional differentiation of implanted myocardium in injured myocardium and to promote the recovery of cardiac function. The curative effect is better than simple stem cell transplantation.