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目的探讨乙型肝炎病毒核酸相关抗原(HBV NRAg)用于临床乙型肝炎诊断的实验价值及与乙型肝炎病毒DNA定量的相关性。方法收集门诊及住院患者标本432份,ELISA法检测血清HBV NRAg、HBV血清标志物,FQ-PCR检测HBV DNA,同时进行肝功能生化检测。结果HBV NRAg与HBV DNA的总符合率为90.70%;86例HBV DNA阳性样本中,HBV NRAg阳性率为95.35%,显著高于PreS1(79.06%)和HBeAg(51.16%),差异有统计学意义(P<0.05)。结论HBV NRAg与HBV DNA具有很好的一致性,可作为临床HBV感染的筛选及判断HBV复制的有意义的补充项目。
Objective To investigate the clinical value of hepatitis B virus nucleic acid associated antigen (HBV NRAg) in the diagnosis of clinical hepatitis B and its relationship with the quantification of hepatitis B virus DNA. Methods 432 samples of outpatients and inpatients were collected, serum HBV NRAg and HBV serum markers were detected by ELISA, HBV DNA was detected by FQ-PCR, and biochemical detection of liver function was performed. Results The overall coincidence rate of HBV DNA and NRAg was 90.70%. The positive rate of HBV NRAg was 95.35% in 86 HBV DNA positive samples, which was significantly higher than that of PreS1 (79.06%) and HBeAg (51.16%), the difference was statistically significant (P <0.05). Conclusion HBV NRAg has good consistency with HBV DNA, which can be used as a screening screening for clinical HBV infection and a meaningful supplementary item for judging HBV replication.