目的：分析绝经后阴道炎的病原菌。方法：对５０例绝经后阴道炎进行细菌培养检查，取患者的子宫颈口分泌物作直接涂片及细菌培养，根据细菌的形态、培养及生化特性进行鉴定。结果：葡萄球菌２０例（占４０％），淋病奈瑟菌１４例（占２８％），阴道加特纳菌１２例（占２４％），假丝酵母菌３例（占６％），大肠埃希菌１例。结论：在绝经后阴道炎中，葡萄球菌感染最常见，淋病奈瑟菌次之；提示定期对绝经后妇女阴道分泌物的培养是必要的。 Objective: To analyze the pathogens of postmenopausal vaginitis. Methods: 50 cases of post-menopausal vaginitis bacterial culture examination, take the patient’s cervical secretions for direct smear and bacterial culture, according to the bacterial morphology, culture and biochemical characteristics were identified. RESULTS: Staphylococcus was found in 20 (40%), Neisseria gonorrhoeae in 14 (28%), Gardnerella vaginalis in 12 (24%), Candida in 3 (6%), Escherichia coli in 1 case. CONCLUSIONS: Staphylococcal infection is the most common form of post-menopausal vaginitis, followed by Neisseria gonorrhoeae; suggesting that regular vaginal secretions are necessary for postmenopausal women.