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探讨共刺激分子在Pristane诱导狼疮鼠体内的表达变化及意义。采用6~8周龄雌性BALB/c鼠单次腹腔注射0.5ml Pristane,对照组注射0.5ml PBS。通过尿蛋白试纸法检测尿蛋白含量;ELISA法检测外周血自身抗体(抗ds-DNA、抗Sm/RNP)含量;HE染色评估病理学损伤;流式细胞术检测小鼠外周血、脾脏淋巴细胞表面共刺激分子CD28、CD40L/CD40、CD137/CD137L的表达;免疫组织化学法检测小鼠组织中共刺激分子CD28、CD40L、CD137的表达。实验结果显示:与对照组小鼠相比,模型组小鼠出现多种SLE样临床症状和体征;模型组小鼠外周血和脾脏淋巴细胞表面共刺激分子CD28,一个月时表达增高,后逐渐下降至低于正常对照组(P<0.05);CD40三个月时表达升高,六个月时表达降低至低于正常对照组(P<0.05),而CD40L的表达变化无统计学意义(P>0.05);CD137/CD137L三个月时表达增高并维持高表达状态至处死(P<0.05);狼疮鼠肺组织中共刺激分子CD28、CD40L和CD137呈现高表达状态,其他组织中变化并不明显。以上结果提示,Pristane诱导狼疮鼠体内共刺激分子表达发生异常变化,可能与其疾病的发生发展有密切关系,为临床治疗系统性红斑狼疮提供了研究基础。
To investigate the changes of costimulatory molecules in Pristane-induced lupus mice and its significance. Female BALB / c mice aged 6-8 weeks were injected intraperitoneally with 0.5 ml of Pristane, while the control group was injected with 0.5 ml of PBS. Urine protein content was detected by urinary protein test paper method; the level of autoantibodies (anti-ds-DNA, anti-Sm / RNP) in peripheral blood was detected by ELISA; pathological damage was evaluated by HE staining; the peripheral blood and spleen lymphocytes were detected by flow cytometry The expression of costimulatory molecules CD28, CD40L / CD40 and CD137 / CD137L were detected by immunohistochemistry. The expression of costimulatory molecules CD28, CD40L and CD137 were detected by immunohistochemistry. The results showed that: Compared with the control group mice, the model group of mice showed a variety of clinical signs and symptoms of SLE-like; the model group of peripheral blood and spleen lymphocyte surface costimulatory molecules CD28 expression increased at one month, gradually (P <0.05). The expression of CD40 increased at the third month and decreased to the level at the sixth month (P <0.05), while the expression of CD40L had no statistical significance (P <0.05) (P <0.05). CD137 / CD137L expression increased and remained high at 3 months (P <0.05). Costimulatory molecules CD28, CD40L and CD137 in lung tissue of lupus mice were highly expressed, but not in other tissues obvious. These results suggest that Pristane induced abnormal changes of co-stimulatory molecules expression in lupus mice may be closely related to the occurrence and development of the disease, providing the basis for the clinical treatment of systemic lupus erythematosus.