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用BCG和牡牛分支杆菌的悬液来吸收血清从而改进了FLA—ABS试验。细菌阳性病人的试验结果几乎100％是阳性,结核样病人约80％是阳性;而18例肺结核病人、50例健康非接触者和138例普通医院非麻风病人中的136例全都是阴性。后者的2例阳性血清与耻垢分支杆菌出现交叉反应,但耻垢分支杆菌不能吸收麻风菌的特异阳性反应。因此证明,用FLA—ABS试验检查麻风菌的临床下感染,其特异性和敏感性都是令人满意的。 62名麻风病人家属接触者中,有57人是阳性(91.9％),其中婴幼儿、病人子女和孙子以及瘤型病人接触者的平均抗体滴度都比较高。家属接触者中有39人作了麻风菌素试验,7人的光田氏反应为可疑,但FLA—ABS试验是阳性,他们显然感染了麻风菌但还未形成细胞免疫能力,因此必须仔细观察,看其是否有发展成为麻风病者。麻风流行区的15000名学校儿童中,有耳大神经粗大或可疑皮疹者共173人作了FLA—ABS试验,阳性有109人(63％)。农村的阳性百分率稍高于城市地区,与过去农村发病率较高的情况相符。FLA—ABS试验的结果与这些儿童的结核菌素试验、BCG接种史无关。他们的血清同时进行了BCG间接免疫荧光检查(BCG—FA)和风疹病毒血凝抑制试验(R—HI),这些试验的结果与FLA—ABS试验结果无相关的关系。FLA—ABS试验与BCG—FA试验都是阳性的58例血清中,57例的血清除了与麻风菌发生反应外,不与另外六种分支杆菌发生反应,有一例同时与另外的分支杆菌发生反应,这个学生后来被发现是麻风病。根据上述的观察推测,该地学校儿童至少是0.7％的临床下感染率,这几乎是当地麻风发病率的200倍。
The FLA-ABS assay was improved by sera from BCG and Mycobacterium bovis suspensions. Almost 100% of the bacteria-positive patients tested positive and about 80% of tuberculosis-like patients were positive, whereas 136 of the non-leprosy patients were negative in 18 tuberculosis patients, 50 healthy non-contact patients and 138 general hospital non-leprosy patients. The latter two cases of positive serum and M. smegmatis cross-reaction, but Mycobacterium smegmatis can not absorb the specific positive reaction of leprosy. Thus, it was demonstrated that the specificity and sensitivity of the leprosy clinical infection with the FLA-ABS test was satisfactory. Totally 57 out of 62 leprosy contacts were positive (91.9%). Among them, the average antibody titers of infants, children, grandchildren and tumor patients were higher. 39 of the family contacts had leprosycin and seven of them were suspicious, but the FLA-ABS test was positive and they were clearly infected with leprosy but did not yet form cellular immunity, so they must be carefully observed, See if they have developed into leprosy. Of the 15,000 schoolchildren living in the endemic area, 173 (63%) had a FLA-ABS test with a total of 173 patients with grossly large or suspected large eardrums. The positive percentage in rural areas is slightly higher than that in urban areas, which is consistent with the high prevalence in rural areas in the past. FLA-ABS test results and these children’s tuberculin test, BCG vaccination history has nothing to do. Their serum was tested for BCG indirect immunofluorescence (BCG-FA) and rubella virus hemagglutination inhibition (R-HI) at the same time. The results of these tests were not related to the FLA-ABS test results. Fifty-eight serum samples with FLA-ABS test and BCG-FA test were positive. In addition to the reaction with leprosy, 57 of the sera did not react with the other six mycobacteria, and one case reacted with another mycobacterium at the same time The student was later found to be leprosy. According to the above observations, it is estimated that at least 0.7% of children in this area have clinically infected rates, which is almost 200 times the incidence of local leprosy.