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本实验选用Wistar新生大鼠,出生后第2天开始腹腔注射葡萄糖或葡萄糖加精氨酸共6天,每日2次,对照组不做处理.分别于生后第10、20天剖腹取胰腺,制作光电镜标本,并用免疫组织化学PAP标记β细胞.同时测定β细胞及其分泌颗粒的数面密度(NA)和体密度(Vv).结果显示:对照组第20天胰β细胞及其分泌颗粒的NA、Vv均明显高于第10天组;尽管实验组β细胞的NA、Vv低于对照组.但其β颗粒的NA、Vv却显著高于对照组。上述结果提示.大鼠生后发育期间胰岛β细胞及其分泌颗粒的数量、体积逐渐增加,而葡萄糖、精氨酸主要引起β细胞提前成熟。
In this experiment, Wistar newborn rats were selected, and intraperitoneal injection of glucose or glucose plus arginine was performed on the second day after birth for 6 days, twice a day, without any treatment in the control group. Pancreatic glands were harvested on the 10th and 20th day after birth, respectively. The specimens were examined with light microscopy and labeled with immunohistochemical PAP. The number density (NA) and density (Vv) of β cells and their secretory granules were measured simultaneously. The results showed that the NA and Vv of pancreatic β cells and their secretory granules on the 20th day in the control group were significantly higher than those on the 10th day. Although the NA and Vv of β cells in the experimental group were lower than those in the control group. However, the NA of particles and Vv of β particles were significantly higher than that of control group. The above results suggest. The quantity and volume of pancreatic β-cells and their secreted granules during the development of rats after birth increased gradually, while the glucose and arginine mainly caused premature maturation of β-cells.