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用含有血液或血红蛋白的琼脂培养基,虽然较易于培养利什曼原虫,但当用于制备抗原时,少量的红细胞或固体碎屑不易从前鞭毛型中除去。作者用液体培养基代替琼脂另加溶血滤液进行培养。液体培养基成分为:
With agar medium containing blood or hemoglobin, while it is easier to culture Leishmania, a small amount of red blood cells or solid debris are less likely to be removed from pre-flagella when used to prepare antigens. The authors used liquid medium instead of agar plus hemolytic filtrate culture. Liquid medium composition: