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目的研究芹菜素对体外人胶质瘤SHG-44细胞增殖抑制和诱导凋亡的作用。方法MTT法检测细胞增殖抑制率。Hoechst 33258和Tunel染色法观察细胞凋亡的形态学变化。AnnexinVFITC/PI双染流式细胞术检测不同浓度芹菜素处理后细胞凋亡率。流式细胞术检测细胞周期。Western blot检测凋亡相关蛋白Bax和Bcl-2的表达变化。结果芹菜素能抑制SHG-44细胞的增殖,并呈时间和剂量依赖性(P<0.05)。芹菜素处理组细胞出现典型细胞凋亡特征。芹菜素可使SHG-44细胞阻滞在G_2/M期。不同浓度的芹菜素作用24h,细胞凋亡率逐渐增加,呈浓度依赖性。芹菜素处理组细胞的Bax蛋白表达量随药物浓度的增加而增加,Bcl-2蛋白表达量随药物浓度的增加而降低。结论芹菜素能抑制胶质瘤细胞增殖,并可通过线粒体信号途径诱导胶质瘤细胞凋亡。
Objective To study the effect of apigenin on proliferation inhibition and apoptosis induction of human glioma SHG-44 cells in vitro. Methods MTT method was used to detect the inhibition rate of cell proliferation. Hoechst 33258 and Tunel staining were used to observe the morphological changes of apoptosis. AnnexinVFITC / PI double staining flow cytometry was used to detect the apoptosis rate of different concentrations of apigenin treatment. Flow cytometry was used to detect cell cycle. Western blot was used to detect the expression of Bax and Bcl-2. Results Apigenin could inhibit the proliferation of SHG-44 cells in a time and dose-dependent manner (P <0.05). Apoptotic characteristics of apigenin-treated cells were observed. Apigenin can arrest SHG-44 cells in G2 / M phase. Different concentrations of apigenin 24h, apoptosis rate increased gradually, in a concentration-dependent manner. The expression of Bax protein in apigenin-treated group increased with the increase of the drug concentration, while the expression of Bcl-2 protein decreased with the increase of the drug concentration. Conclusion Apigenin can inhibit glioma cell proliferation and induce apoptosis of glioma cells through mitochondrial signaling pathway.