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目的 :研究小鼠急性中度骨髓型放射损伤细胞免疫功能的变化及养阴抗毒散的防护作用 .方法 :以 6GyX射线照射小鼠为动物模型 ,随机分为正常对照组 (A)、照射组 (B)及照射加中药组 (C) .用流式细胞仪直接免疫荧光染色法测定外周血T淋巴细胞亚群 ,酵母菌红细胞花环试验法测定红细胞免疫能力 .结果 :与A组比较 ,B ,C两组小鼠CD4 ,CD8百分率显著减少 (P <0 .0 1 ) ;B组CD4 /CD8比值明显降低 (P<0 .0 1 ) .B组小鼠RBC C3 bR花环率第 1 2 ,2 0 ,2 8日降低 (P<0 .0 1 ) ,RBC IC花环率第 4日和第 8日升高 (P <0 .0 1 ) ;C组小鼠RBC C3 bR花环率仅在第 1 2日下降 (P <0 .0 1 ) ,RBC IC花环率仅在第 4日升高 (P <0 .0 1 ) ;C组RBC C3 bR花环率在第 4 ,1 2 ,2 0 ,2 8日均高于B组 (P <0 .0 1 ) .结论 :6GyX线照射可造成小鼠细胞免疫功能低下 ,养阴抗毒散对此有一定的防治作用 .
OBJECTIVE: To study the changes of cellular immune function in mice with acute moderate bone marrow-type radiation injury and the protective effects of nourishing yin and anti-toxic drugs. METHODS: The mice were irradiated with 6 Gy X-rays and randomly divided into normal control group (A) and irradiation. Group (B) and Irradiation plus Chinese Herbal Medicine (C). Peripheral blood T lymphocyte subsets were measured by flow cytometry with direct immunofluorescent staining, and red blood cell immunity assays were performed using the yeast red cell rosette assay. Results: Compared with group A, The CD4 and CD8 percentages of mice in groups B and C were significantly decreased (P < 0.01), and the CD4/CD8 ratio in group B was significantly lower (P <0.01). The RBC C3 bR rosette rate in group B was the 1st. Decreased on days 2, 20, and 28 (P<0. 01), RBC IC rosette rate increased on day 4 and day 8 (P < 0.01); C group mouse RBC C3 bR rosette rate only Decreased on the 12th day (P <0. 01), RBC IC rosette rate only increased on the 4th day (P <0. 01); C group RBC C3 bR rosette rate on the 4th, 1 2nd, 2nd On day 0 and day 28, they were all higher than those in group B (P < 0.01). Conclusion: 6Gy X-ray irradiation can cause low cellular immune function in mice. Yangyin Kangdusan can prevent and cure this effect.