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目的:研究急性坏死性胰腺炎(ANP)大鼠24h内脑脊液和血清髓鞘碱性蛋白(MBP)水平的动态变化。方法:分别诱导SD大鼠ANP和急性水肿性胰腺炎(AEP)模型,同时设立假手术组。分别于模型诱导后3、6、9、12、24h抽取小脑延髓池脑脊液和腹腔动脉血液。以ELISA法测定脑脊液、血清中MBP的含量。透射电镜观察髓鞘超微结构变化。结果:ANP组大鼠诱导后3h,脑脊液MBP水平明显高于AEP组和假手术组(P<0.01),6、9h时仍维持在较高水平(P<0.05),12、24h时则降至较低水平。ANP组、AEP组和假手术组血清MBP水平无显著差异(P>0.05),在24h内维持于较低水平。电镜检查,3～9hANP组脑髓鞘主要呈轻度的损伤性改变,如空泡化、板层结构散乱等改变。结论:大鼠ANP早期存在脑组织髓鞘结构损害,表现为脑脊液MBP水平明显升高。
Objective: To study the dynamic changes of cerebrospinal fluid and serum MBP in acute necrotizing pancreatitis (ANP) rats in 24h. Methods: ANP and acute edematous pancreatitis (AEP) model were induced in SD rats and sham operation group was established. Cerebrospinal fluid and celiac artery blood were collected from the cisterna magna at 3, 6, 9, 12 and 24 hours after model induction. The contents of MBP in CSF and serum were measured by ELISA. TEM observation of ultrastructure changes of myelin. Results: The level of MBP in cerebrospinal fluid in ANP group was significantly higher than that in AEP group and sham operation group at 3h after induction (P <0.01), and remained high at 6 and 9h (P <0.05) To a lower level. There was no significant difference in serum MBP levels between ANP group, AEP group and sham operation group (P> 0.05), and maintained at a lower level within 24h. Electron microscopy, 3 ~ 9hANP group of cerebral myelin lesions showed mild changes, such as vacuolization, scattered changes in the lamellar structure. Conclusion: Myelin structure damage of brain tissue exists in ANP rats at early stage, which shows that the level of MBP in cerebrospinal fluid is obviously increased.