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目的：明确在乳腺癌组织和细胞中Delta受体的分布情况，并验证其在乳腺癌细胞MCF-7增殖进展中的作用。方法：收集乳腺癌组织标本并培养人乳腺细胞和人乳腺癌细胞，通过逆转录聚合酶链反应（RT-PCR）检测DORmRNA在乳腺癌组织及细胞中的变化。统计分析DOR表达与临床病理特征间的关系。MTT法测定并对比病毒转染组、空白对照组和阴性对照寡核苷酸组细胞活力；流式细胞仪测定三组细胞周期。结果：DORmRNA在乳腺癌组织及乳腺癌细胞中表达明显高于相应的癌旁组织及正常乳腺细胞（p<0.05）。DOR的表达与肿瘤转移及临床分期呈正比。转染组细胞活性明显低于空白对照组和阴性对照寡核苷酸组，且转然后大部分肿瘤细胞停留在G1期，具有显著差异。结论：DOR广泛表达在乳腺癌组织和细胞中，且与乳腺癌的发展密切相关。“,”Objective:To define the distribution of Delta receptor in breast cancer tissues and cels, and to verify the role of MCF-7 receptor in the progression of breast cancer cel proliferation.Methods:Breast cancer tissue samples were colected and human breast cancer cels and human breast cancer cels were cultured. By reverse transcription polymerase chain reaction (RT-PCR), the changes of DORmRNA in breast cancer tissues and cels were detected. Statistical analysis of the relationship between DOR expression and clinical pathological characteristics.MTT method was used to measure and compare the cel viability of the virus transfection group, the blank control group and the negative control group. The cel cycle was determinedby flow cytometry in three groups.Results:The expression of DORmRNA in breast cancer tissues and breast cancer cels was significantly higher than that in the corresponding adjacent tissues and normal breast cels (p<0.05). The expression of DOR was directly proportional to tumor metastasis and clinical stage. The cel activity in transfected group was significantly lower than that in the control group and the negative control group, and then most of the tumor cels remained in the G1 phase, which had significant difference. Conclusions:DOR is widely expressed in breast cancer tissues and cels, and is closely related to the development of breast cancer.