用眼内高压法造成大鼠视网膜缺血45min，然后回复常压，造成缺血-再灌注损伤。采用符合临床给药途径的眼球分注射法，小鼠颌下腺神经生长因子（NGF）250、500、1000BU／眼，于缺血损伤前及其后球旁注射，每天1次，连续4天，第10天取材病检。观察到NGF500、1000BU／眼可减轻视网膜缺血-再灌注损伤，使神经节细胞存活率增加，视网膜全层及内核层萎缩减轻。提示NGF球旁注射可扩散到视网膜，对视网膜缺血-再灌注损伤产生保护作用。 Intraocular pressure was used to induce retinal ischemia in rats for 45 min and then returned to normal pressure, resulting in ischemia-reperfusion injury. The eyeballs were injected subcutaneously in the submandibular gland at a dose of 250,500,1000BU / mouse in the submandibular gland. The mice were injected with the ball at the anterior and posterior sides of the ball, once a day for 4 days. 10 days of material disease inspection. Observed NGF500, 1000BU / eye can reduce retinal ischemia-reperfusion injury, so that increased survival of ganglion cells, retinal layer and the atrophy of the inner nuclear layer to reduce. Tip NGF ball injection can diffuse into the retina, retinal ischemia-reperfusion injury have a protective effect.