着重介绍视网膜神经节细胞 (RGCs)的培养、分离、鉴定及影响因素等方面的新进展。RGCs的培养分为视网膜外植块培养、分离的RGCs细胞培养。培养基有含血清及无血清两种。在作RGCs培养前 ,需对培养皿、培养瓶等进行处理。在细胞鉴定方面 ,采用活细胞观察、Thy 1抗体标记、荧光素标记、免疫组化标记等方法。影响RGCs培养的因素有上丘提取液、温度、胶质细胞层及缺氧、兴奋性毒素等。 It focuses on the new progress in the culture, isolation, identification and influential factors of retinal ganglion cells (RGCs). The culture of RGCs was divided into retinal explant culture and isolated RGCs cell culture. Medium with serum and serum-free two. For RGCs before training, Petri dishes, flasks, etc. need to be processed. In cell identification, using live cell observation, Thy 1 antibody labeling, fluorescein labeling, immunohistochemistry and other methods. Affect the RGCs cultured factors are the superior colliculus extract, temperature, glial cell layer and hypoxia, excitotoxicity and so on.