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以可溶性淀粉为原料,利用反向微乳液法,加入交联剂三氯氧磷,制备了直径为 50 nm左右带负电荷的交联淀粉纳米颗粒. 以该纳米颗粒为内核,经多聚赖氨酸修饰,得到了多聚赖氨酸淀粉纳米颗粒(PLL-StNP). 对 PLL-StNP 进行了颗粒粒度、稳定性和电性的表征,并通过颗粒的体外细胞毒性检测、颗粒与 DNA 结合能力及细胞转染等方面的分析,发现多聚赖氨酸淀粉纳米颗粒(PLL-StNP)有可能作为基因载体,在此基础上发展了多聚赖氨酸淀粉纳米颗粒基因载体的构建与基因转染技术. 作为非病毒基因载体,赖氨酸淀粉纳米颗粒具有基因装载量大、转染率高、细胞毒性低以及可生物降解等优点.
Soluble starch as raw material, the use of reverse microemulsion method, adding cross-linking agent phosphorus oxychloride, a diameter of about 50 nm negatively charged cross-linked starch nanoparticles with the nanoparticles as the core, the poly Lai (PLL-StNP) .Particle-size, stability and electrical characterization of PLL-StNP were carried out and the particles were bound to DNA through the in vitro cytotoxicity test Ability and cell transfection and other aspects of the analysis and found that poly-lysine starch nanoparticles (PLL-StNP) may be used as a gene carrier, on the basis of the development of polylysine starch nanoparticle gene vector construction and gene Transfection technology As a non-viral gene carrier, lysine starch nanoparticles with gene loading capacity, high transfection efficiency, low cytotoxicity and biodegradability advantages.