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目的 :探讨创伤应激状态下小鼠细胞免疫功能变化及黄芪多糖 (APS)对其影响。方法 :采用小鼠截肢应激模型 ,将 5 0只Balb/c小鼠随机分为 5组 :正常对照组 (A) ;应激对照组(B) ;应激 +APS高 (C)、中 (D)、低 (E)剂量组 ,每组 10只。免疫组织化学技术检测小鼠胸腺、脾脏组织中CD4 、CD8、c -fos蛋白表达 ,计算机图像分析系统定量。结果 :与正常对照组 (A)比较 ,创伤后 72小时应激对照组 (B)小鼠CD4 抗原分布、CD4 /CD8比值显著减少 (P<0 0 1) ,c -fos抗原分布明显多于正常 (P <0 0 1) ;与创伤应激对照组 (B)比较 ,应激 +APS高 (C)、中 (D)、低 (E)剂量组小鼠CD4抗原水平、CD4 /CD8比值升高 (P <0 0 1)、c-fos抗原水平降低 (P <0 0 1) ;与正常对照组 (A)比较 ,应激 +APS高剂量组 (C)小鼠CD4、CD8、c -fos抗原分布无显著差异 (P >0 0 5 )。结论 :创伤后小鼠细胞免疫功能紊乱 ;黄芪多糖可有效恢复其细胞免疫功能。
Objective: To investigate the changes of cellular immune function in mice with traumatic stress and the effect of APS on it. METHODS: Using a mouse amputation stress model, 50 Balb/c mice were randomly divided into 5 groups: normal control group (A); stress control group (B); stress + APS high (C), middle (D) Low (E) dose groups of 10 per group. The expression of CD4, CD8 and c-fos protein in mouse thymus and spleen tissues was detected by immunohistochemical technique and quantified by computer image analysis system. RESULTS: Compared with the normal control group (A), the CD4 antigen distribution, CD4/CD8 ratio, and CD4/CD8 ratio were significantly reduced in the stress control group (B) at 72 hours post-injury (P<0 01), and the c-fos antigen distribution was significantly greater than Normal (P <0 01); Compared with traumatic stress control group (B), CD4 antigen level and CD4/CD8 ratio in mice with stress + APS high (C), medium (D), and low (E) doses Elevated (P <0 01), decreased c-fos antigen level (P <0 01); Compared with normal control group (A), stress + APS high-dose group (C) mice CD4, CD8, c There was no significant difference in the distribution of -fos antigens (P > 0 05). Conclusion : The cellular immune function of mice after trauma is disturbed. Astragalus polysaccharide can effectively restore its cellular immune function.