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用EMS诱变籼型水稻(Oryza sativa)恢复系缙恢10号,获得稳定遗传的脆性叶尖枯死突变体fld1,苗期植株呈现脆性和叶尖枯死,机械强度显著下降,一直持续到成熟。突变体fld1茎秆中的纤维素和木质素含量仅分别为野生型的67.92%和50.16%,差异达极显著水平。与野生型相比,fld1叶片中的光合色素含量呈现一定程度的下降。其中,类胡萝卜素含量在衰老和正常部位均极显著降低,净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)也极显著降低,胞间CO2浓度(Ci)则极显著升高。遗传分析表明,fld1的脆性和叶尖枯死性状共分离,且受1对隐性核基因调控。利用西农1A/fld1和fld1/日本晴的F2群体,最终将FLD1定位在第9染色体Indel标记Ind09-2与Ind09-3之间215 kb的物理距离内,包含33个注释基因。研究结果为下一步基因的克隆和功能研究奠定了基础。
The mutants of frydiploid mutant fld1, which had a stable genetic inheritance, were obtained by EMS mutagenesis of Jinhui 10, an indica rice (Oryza sativa). The plants showed brittle and withered leaves withered at the seedling stage, and the mechanical strength decreased markedly and continued until maturity. The content of cellulose and lignin in the mutant fld1 was only 67.92% and 50.16% of the wild type, respectively, with a significant difference. Compared with the wild-type, fld1 photosynthetic pigment content in leaves showed a certain degree of decline. The contents of carotenoids decreased significantly at both senescence and normal sites, and Pn, Gs and Tr also decreased significantly. The intercellular CO2 concentration (Ci) was extremely significant Rise. Genetic analysis showed that fld1 fragmentation and leaf tip dead traits co-segregation, and by a recessive nuclear gene regulation. Using the F2 population of Xiunong 1A / fld1 and fld1 / Nipponbare, FLD1 was eventually mapped within 215 kb of the physical distance between Indel markers Ind09-2 and Ind09-3 on chromosome 9, containing 33 annotation genes. The results laid the foundation for the next gene cloning and functional studies.