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目的 :研究PGE1在脊髓水平对不同性质疼痛的调控作用。方法 :清醒小鼠鞘内注射法给药 ,用热水缩尾法 ,福尔马林实验 ,冰醋酸扭体法等三种方法测痛。结果 :在热痛模型上 ,PGE10 .1ng～ 0 .1 μg/只使小鼠痛阈显著降低 ,1 0ng时最明显 ,而在较大剂量 1 μg/只无明显作用。福尔马林实验中 ,PGE10 .1 ,1 0ng/只无显著作用 0 .1 μg/只抑制其I相反应 ,1 μg/只显著抑制其Ⅰ、Ⅱ相反应。在冰醋酸扭体模型中PGE10 .1 ,1 0ng/只无明显效果。 0 .1 ,1 μg/只降低小鼠扭体次数呈镇痛作用。结论 :PGE1在脊髓对不同性质疼痛调控作用有本质差别 ,即对热痛有易化作用 ,而对外周神经直接化学性刺激和内脏炎性痛有镇痛作用
PURPOSE: To study the regulation of PGE1 on spinal cord injury to different types of pain. Methods: Intrathecal injection of awake mice, hot-water tailing method, formalin test, acetic acid writhing and other three methods to measure pain. Results: In the heat pain model, PGE10 .1ng ~ 0. 1μg / mouse pain threshold was significantly reduced, the most obvious when 10ng, but at a higher dose of 1μg / only no significant effect. Formalin test, PGE10. 1, 10ng / no significant effect of 0.1μg / only inhibit its phase I response, 1μg / only significantly inhibited its phase Ⅰ, Ⅱ reaction. In glacial acetic acid writhing model PGE10. 1, 10ng / no significant effect. 0 .1, 1 μg / mouse writhing reduce the number of analgesic effect. CONCLUSION: PGE1 plays an essential role in the regulation of pain in different types of spinal cord, that is, it has facilitation of thermal pain and analgesic effect of direct chemical stimulation of peripheral nerves and visceral inflammatory pain