淋巴细胞转化验试不仅是测定机体免疫状态的较好方法,也是研究细胞生物学,免疫学药理学的良好指标。为了阐明活血化瘀药作用原理我们观察了川芎嗪等药物对体外培养的淋巴细胞DNA合成的影响,以此作为细胞免疫功能的监测指标,以了解川芎嗪等活血化瘀药对机体免疫机能的影响。材料和方法我们采集的血样系健康成人血,采用微量全血放射性同位素测定法,实验程序可概括为: 采血→全血加至Eagle氏培养液中(?)同位素掺入(?)终止培养,收获制样→同位素测定。(1)淋巴细胞培养:新采集的肝素抗凝全血0.1毫升接种在含有抗菌素的体积为3毫升的培养基中(每毫升营养液含100单位青霉素和100微克链霉索)每并加入0.15毫升、0.2%的PHA(上海东风试剂厂出品)。每组至少 The lymphocyte transformation test is not only a good method for measuring the immune status of the body, but also a good indicator for studying cell biology and immunological pharmacology. In order to elucidate the principle of action of blood circulation and phlegm-resolving drugs, we observed the effects of ligustrazine and other drugs on the DNA synthesis of lymphocytes cultured in vitro as a monitoring index of cellular immune function to understand the effects of ligustrazine and other blood circulation drugs on immune function of the body. influences. Materials and Methods The blood samples collected were healthy adult human blood samples. Radioactive isotope assay was used for trace amounts of whole blood. The experimental procedure can be summarized as follows: Blood sampling → Whole blood was added to Eagle’s medium (?) Isotope incorporation (?) Terminated culture, Harvest samples → isotope determination. (1) Lymphocyte culture: 0.1 ml of newly collected heparin anticoagulant whole blood is inoculated in a medium containing antibiotics in a volume of 3 ml (containing 100 units of penicillin and 100 micrograms of streptoline per ml of nutrient solution) Ml, 0.2% PHA (Shanghai Dongfeng Reagent Factory). At least each group