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目的检测重度肥胖症患者膳食干预过程中,肠道内硫酸盐还原菌(SRB)的数量变化,为进一步研究SRB与肥胖的关系提供参考。方法以SRB基因组中的重要功能基因-腺苷酰硫酸(APS)还原酶基因作为指示基因,通过实时定量PCR的方法检测了12名重度肥胖症患者在进行膳食干预过程中随着体重的降低,肠道内SRB的数量变化,同时以16S rRNA基因对肠道内总菌进行定量来计算SRB占总菌的相对比例。结果膳食干预过程中,随着患者体重的显著降低,其肠道内SRB占总菌的比例也显著下降。在维持期,患者体重仍和干预前有显著差异,但较干预期有所回升;其体内SRB含量也显著低于干预前,但相比干预期,有所上升。结论研究结果提示肠道菌群中SRB细菌与肥胖的发展有密切关系,为后续研究SRB细菌的功能和作用机理奠定了基础。
Objective To detect the changes of the numbers of sulphate-reducing bacteria (SRB) in the diet of patients with severe obesity during the course of dietary intervention, so as to provide references for further study on the relationship between SRB and obesity. Methods Using the gene of APS reductase, an important functional gene in SRB genome, as an indicator gene, 12 severe obesity patients were tested for their weight loss during dietary intervention by real-time PCR. The number of SRB in the intestine was varied, and the relative proportion of SRB in the total bacteria was calculated by quantifying the total intestinal bacteria with 16S rRNA gene. Results During dietary intervention, the proportion of SRB in the intestine to total bacteria decreased significantly with the significant decrease in body weight. During the maintenance phase, the body weight of the patients remained significantly different from that before the intervention, but it recovered from the intervention period. The SRB content of the patients was also significantly lower than that before the intervention, but increased compared with the intervention period. Conclusions The results suggest that SRB bacteria in intestinal flora are closely related to the development of obesity, which lays the foundation for further studies on the function and mechanism of SRB bacteria.