论文部分内容阅读
目的探讨西罗莫司对恶性淋巴瘤细胞的作用及其可能机制。方法实验分为西罗莫司组、阿霉素组、西罗莫司+阿霉素组、对照组,观察各组人非霍奇金淋巴瘤Raji细胞增殖、凋亡及细胞周期分布情况,应用MTT比色法检测细胞生长情况,流式细胞术(FCM)分析细胞凋亡及细胞周期分布的情况;应用FCM和免疫细胞化学染色法检测HIF-1α蛋白在西罗莫司各组中的表达情况。结果(1)西罗莫司和阿霉素对Raji细胞生长具有抑制作用,且在一定范围内呈时间剂量依赖性;(2)西罗莫司+阿霉素组吸光度值(A492)与西罗莫司组、阿霉素组相比差异均有显著性(P<0.05);(3)西罗莫司+阿霉素组凋亡率与对照组、阿霉素组相比差异均有显著性(P<0.05),与西罗莫司组相比差异无显著(P>0.05);(4)西罗莫司+阿霉素组各期细胞比例与对照组、阿霉素组相比,细胞周期分布发生明显变化(P<0.05),与西罗莫司组差异无显著性(P>0.05)。(5)西罗莫司各浓度组HIF-1α蛋白表达率下降,与对照组相比差异有显著性(P<0.05)。结论西罗莫司体外对Raji细胞生长具有明显的抑制作用。西罗莫司通过阻滞Raji细胞G0/G1期抑制Raji细胞增殖,通过下调HIF-1α蛋白的表达诱导Raji细胞凋亡。西罗莫司与阿霉素联合对Raji细胞生长具有明显抑制作用,优于单用西罗莫司,但对Raji细胞凋亡和周期的影响与单用西罗莫司无显著差异。
Objective To investigate the effect of sirolimus on malignant lymphoma cells and its possible mechanism. Methods The experiment was divided into three groups: sirolimus group, doxorubicin group, sirolimus + doxorubicin group and control group. The proliferation, apoptosis and cell cycle distribution of Raji cells in non-Hodgkin’s lymphoma of each group were observed, Cell growth was detected by MTT colorimetric assay. Cell apoptosis and cell cycle distribution were analyzed by flow cytometry (FCM). FCM and immunocytochemical staining were used to detect the expression of HIF-1α in sirolimus Express the situation. Results (1) Sirolimus and doxorubicin inhibited the growth of Raji cells in a dose-and time-dependent manner. (2) The absorbance values of sirolimus and doxorubicin group (A492) (P <0.05). (3) The apoptosis rates of sirolimus and doxorubicin groups were significantly lower than those of the control and doxorubicin groups (P <0.05), but no significant difference compared with sirolimus group (P> 0.05). (4) The percentage of cells in sirolimus and doxorubicin groups was significantly higher than that in control group, doxorubicin group Compared with the sirolimus group, there was no significant difference (P> 0.05). (5) The expression of HIF-1α in sirolimus concentration group decreased significantly compared with the control group (P <0.05). Conclusion Sirolimus can inhibit the growth of Raji cells in vitro. Sirolimus can inhibit the proliferation of Raji cells through arresting the G0 / G1 phase of Raji cells and induce the apoptosis of Raji cells by down-regulating the expression of HIF-1α protein. The combination of sirolimus and doxorubicin significantly inhibited the growth of Raji cells, which was superior to that of sirolimus alone. However, the effects of sirolimus and doxorubicin on apoptosis and cycle of Raji cells were not significantly different from that of sirolimus alone.