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为探讨MYB基因在甜樱桃(Prunus avium L.)果实着色过程中的作用,采用RT-PCR克隆得到甜樱桃的一个MYB基因,Pac MYBA,其c DNA全长为946 bp,该段全长序列包含的开放阅读框(ORF)为687 bp,编码228个氨基酸的蛋白质,该蛋白相对分子质量为26.4 k D,等电点为9.0,是不稳定性蛋白,具有强亲水性,在N端有2个MYB DNA结合域。通过实时荧光定量PCR分析结果发现,Pac MYBA在甜樱桃中的表达水平与果实总花青素含量呈正相关,二者均在果实转色阶段后期维持较高的水平,推测Pac MYBA基因在调控“红灯”甜樱桃果实花青素合成起着重要作用。
In order to investigate the role of MYB gene in the fruit coloration of sweet cherry (Prunus avium L.), a MYB gene, Pac MYBA, was cloned by RT-PCR and its full-length c DNA was 946 bp. The open reading frame (ORF) contained was 687 bp, encoding a protein of 228 amino acids. The relative molecular mass of this protein was 26.4 kD and its isoelectric point was 9.0. It was an unstable protein with strong hydrophilicity. There are 2 MYB DNA binding domains. The results of real-time PCR showed that the expression level of Pac MYBA in sweet cherry was positively correlated with the total anthocyanin content in fruit, both of which maintained a high level in the later stage of fruit color conversion. It was speculated that Pac MYBA gene was regulated in “Red light” sweet cherry fruit anthocyanin synthesis plays an important role.