论文部分内容阅读
目的：观察侧脑室移植脑脊液诱导的骨髓源性神经样细胞（BMSC-Ns）对脊髓损伤大鼠BBB评分及神经营养因子分泌的影响。方法全骨髓贴壁法分离培养大鼠骨髓间充质干细胞（BMSCs），人脑脊液诱导得到BMSC-Ns；钳夹法制作SD大鼠脊髓损伤模型，将造模后脊髓损伤大鼠随机分为A、B、C组，每组18只。采用侧脑室注射途径将细胞移植入大鼠体内，A组移植BMSC-Ns（1×106/20μl），B组移植BMSCs（1×106/20μl），C组侧脑室注射等体积生理盐水。于细胞移植前和细胞移植后第1、2、3、4、5、6周对脊髓损伤大鼠进行BBB评分；于细胞移植后第1、4周通过ELISA法测定脑脊液中脑源性神经营养因子（BDNF）、神经生长因子（NGF）、神经营养因子-3（NT-3）的浓度。结果在移植后的各个时间点，A组脊髓损伤大鼠的BBB评分显著高于B组和C组，B组的BBB评分又显著高于C组，差异有统计学意义（P<0.05）。不同时间点亚组间比较显示：细胞移植后前3个时间点A组和B组BBB评分呈现逐渐上升的趋势，亚组间比较差异有统计学意义（P<0.05）；第3周后上升趋势平缓，各时间点BBB评分差异无统计学意义（P＞0.05）。细胞移植后A组、B组大鼠脑脊液中BDNF、NGF、NT-3含量明显高于C组；A组又高于B组，差异均有统计学意义（P＜0.05）。结论侧脑室移植BMSC-Ns和BMSCs可明显改善脊髓损伤大鼠神经功能，并且可以促进脑脊液中BDNF、NGF、NT-3的分泌，BMSC-Ns效果优于BMSCs。“,”Objective To observe the effect of bone marrow-derived neural like cells (BMSC-Ns) induced by cerebrospinal fluid (CSF) on BBB score and secretion of neurotrophic factor in rats with spinal cord injury (SCI). Methods The whole bone marrow adherence method was used to isolate and culture BMSCs. BMSC-Ns were induced by human CSF. The clamping method was used to establish the SCI model, after modeling SCI rats were randomly divided into groups A, B, and C (n=18 each). By intracerebroventricular injection, BMSC-Ns (1 × 106/20 μl) were transplanted into SCI rats in group A, BMSCs (1×106/20μl) in group B, normal saline (1×106/20μl) in group C. Prior to the transplantion, on the first, second, third, fourth, fifth, and sixth week after transplantion, BBB score was used to evaluate the recovery of neurological function. At the first week and the fourth week after transplantion, ELISA was used to measure concentration of brain-derived neural nutrition factor (BDNF), nerve growth factor (NGF), and neurotrophin-3 (NT-3) in CSF. Results At each time point after transplantation, BBB scores of SCI rats in group A was significantly higher than that in group B and group C, the BBB score in group B was significantly higher than that in group C, the difference was statistically significant (P<0.05). At the first three time points after cell transplantation, BBB score of SCI rats in group A and group B showed a gradual upward trend, the difference between different time subgroups was statistically significant (P 0.05). After cell transplantation, the concentration of BDNF, NGF, and NT-3 in CSF of SCI rats in group A and group B was significantly higher than in group C;that in group A was higherthan in group B, the differences were statistically significant (P<0.05). Conclusion Transplantation of both BMSC-Ns and BMSCs through lateral ventricle can significantly improve the neurological function of SCI rats and promote the secretion of BDNF, NGF, and NT-3 into CSF. The effect of BMSC-Ns is better than that of BMSCs.