论文部分内容阅读
目的采用高效液相色谱法测定溃疡宁片中盐酸普鲁卡因的含量。方法色谱柱Luna C18(4.6×250 mm,5μ);流动相:流动相为甲醇-0.4%磷酸与0.4%三乙胺混合溶液(13:87);检测波长为292 nm。结果盐酸普鲁卡因进样量在0.0364μg~0.32976μg范围内峰面积积分值与进样量呈良好的线性关系(r=0.99998),平均加样回收率98.9%,RSD=0.25%(n=6)。结论该方法易于操作,结果准确,重现性良好,可用于溃疡宁片的质量控制。
Objective To determine the content of procaine hydrochloride in Kuiyining Tablets by high performance liquid chromatography. Methods Column Luna C18 (4.6 × 250 mm, 5μ) was used as the mobile phase. The mobile phase consisted of methanol-0.4% phosphoric acid and 0.4% triethylamine mixed solution (13:87). The detection wavelength was 292 nm. Results The peak area integral value of procaine hydrochloride in the range of 0.0364μg ~ 0.32976μg showed a good linear relationship with the injection volume (r = 0.99998), the average recovery was 98.9% and the RSD was 0.25% (n = 6). Conclusion The method is easy to operate, with accurate results and good reproducibility. It can be used for the quality control of Huayining Tablets.