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目的观察活血化瘀中药对兔创伤性膝骨关节炎模型关节液中IL-6及TNF-α含量的影响,探讨活血化瘀法治疗创伤性膝骨关节炎的作用机制。方法新西兰兔48只,按随机数表随机分成3组:中药组,模型制动组,假手术组,阳性药物对照组,每组12只。造模术后4周行右膝关节X片检查,确认造模成功后,中药组灌服活血化瘀中药2.6g/kg(相当于成人用药量当归、赤芍、姜黄各10g),每天2次,连续喂服4周。阳性药物对照组予硫酸氨基葡萄糖片15mg/kg灌服,每日3次,连续灌服4周。假手术组给予等量生理盐水灌胃,每日2次,共4周。4周后处死观察膝关节软骨肉眼形态,并检测关节液中IL-6及TNF-α的含量。结果 1模型组与空白组比较,差异具有统计学意义(P<0.05),说明造模成功。中药组、阳性药物对照组与模型组比较,差异具有统计学意义(P<0.05),说明中药组、阳性药物对照组治疗有效。中药组与阳性药物对照组比较,差异无统计学意义(P>0.05)。2关节软骨中IL-6及TNF-α的检测:模型组与假手术组比较,IL-6及TNF-α表达显著提高(P<0.01);中药组、阳性药物对照组与模型组比较,IL-6及TNF-α显著降低(P<0.01);中药组与阳性药物对照组比较,IL-6及TNF-α无显著性差异(P>0.05)。结论 1KOA模型关节液中IL-6及TNF-α异常升高,说明IL-6及TNF-α与KOA发病有关;2中药组、阳性药物对照组对KOA均有有治疗作用,可降低关节液中IL-6及TNF-α的含量,且两者间无统计学差异。3活血化瘀中药通过抑制关节液中IL-6及TNF-α等炎症因子,从而有效保护膝骨关节炎模型兔关节软骨,延缓关节软骨退变。
Objective To observe the effect of traditional Chinese medicine of promoting blood circulation and removing blood stasis on the content of IL-6 and TNF-α in synovial fluid of rabbit model of traumatic knee osteoarthritis and to explore the mechanism of activating blood circulation and removing blood stasis to treat traumatic knee osteoarthritis. Methods 48 New Zealand white rabbits were randomly divided into 3 groups according to random number table: Chinese medicine group, model brake group, sham operation group and positive drug control group, with 12 rats in each group. Four weeks after the operation, the right knee joint X-ray examination was performed to confirm that the Chinese medicine group was given 2.6g / kg traditional Chinese medicine (equivalent to the adult dosage of angelica root, red peony root and turmeric) Times, continuous feeding for 4 weeks. Positive control group to glucosamine sulfate tablets 15mg / kg orally, 3 times a day for 4 weeks. Sham operation group was given the same amount of saline gavage twice daily for 4 weeks. After 4 weeks, the morphology of the articular cartilage was observed and the levels of IL-6 and TNF-α in the synovial fluid were measured. Results 1 The model group compared with the blank group, the difference was statistically significant (P <0.05), indicating successful modeling. The difference between the traditional Chinese medicine group and the positive medicine control group and the model group was statistically significant (P <0.05), indicating that the traditional Chinese medicine group and the positive drug control group were effective. There was no significant difference between the traditional Chinese medicine group and the positive control group (P> 0.05). The detection of IL-6 and TNF-α in articular cartilage: The expression of IL-6 and TNF-α in model group and sham operation group were significantly increased (P <0.01); Compared with model group, IL-6 and TNF-α significantly decreased (P <0.01). There was no significant difference between IL-6 and TNF-α in TCM group and control group (P> 0.05). Conclusions The abnormal increase of IL-6 and TNF-α in synovial fluid of 1KOA model indicates that IL-6 and TNF-α are related to the pathogenesis of KOA. 2 Chinese medicine group and positive drug control group have therapeutic effect on KOA, In IL-6 and TNF-α content, and no significant difference between the two. 3 Huoxue Huayu traditional Chinese medicine by inhibiting synovial fluid IL-6 and TNF-α and other inflammatory factors, thus effectively protecting the articular cartilage of knee osteoarthritis model rabbits, delaying the degeneration of articular cartilage.