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目的研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)受体在胰腺癌中的表达及意义。方法应用半定量RT-PCR法检测TRAIL受体(死亡受体DR4、DR5和诱骗受体DcR1、DcR2)mRNA在胰腺癌组织及正常胰腺组织中的表达。结果死亡受体DR4和DR5在所有胰腺癌组织和正常胰腺组织中均有表达,且在胰腺癌组织中的表达明显强于在正常胰腺组织中的表达(P<0.01)。诱骗受体DcR1和DcR2在所有正常胰腺组织中均有表达,而在胰腺癌组织中仅有18例表达DcR1,有20例表达DcR2;诱骗受体DcR1和DcR2的表达水平在胰腺癌和正常胰腺组织中差异无统计学意义(P>0.05)。胰腺癌组织中DR5的表达与肿瘤的分化程度和临床分期有关,分化程度越低,DR5的表达量越低,Ⅲ、Ⅳ期肿瘤DR5的表达显著低于Ⅰ、Ⅱ期(P<0.05)。DR4、DcR1及DcR2在胰腺癌组织中的表达与肿瘤的分化程度和临床分期无关(P>0.05)。结论①胰腺癌组织中普遍存在TRAIL受体的表达,并存在受体类型的表达差异,TRAIL基因受体在胰腺癌凋亡的调控机理中可能发挥重要作用。②胰腺癌组织中DR5的表达与肿瘤的分化程度及恶性程度相关;死亡受体DR4及诱骗受体DcR1和DcR2不能作为判断胰腺癌分化程度及恶性程度的指标。
Objective To study the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor in pancreatic cancer and its significance. Methods The expression of TRAIL receptor (DR4, DR5, DcR1, DcR2) in pancreatic cancer and normal pancreas was detected by semi-quantitative RT-PCR. Results The death receptors DR4 and DR5 were expressed in all pancreatic cancer tissues and normal pancreatic tissues, and were significantly higher in pancreatic cancer tissues than in normal pancreatic tissues (P <0.01). The decoy receptors DcR1 and DcR2 were expressed in all normal pancreatic tissues, whereas in pancreatic cancer only 18 cases expressed DcR1 and 20 cases expressed DcR2. The expression levels of decoy receptors DcR1 and DcR2 were significantly higher in pancreatic cancer and normal pancreas The difference was not statistically significant (P> 0.05). The expression of DR5 in pancreatic cancer tissues was related to the degree of differentiation and clinical stage of the tumor. The lower the degree of differentiation, the lower the expression of DR5. The expression of DR5 in stage Ⅲ and Ⅳ was significantly lower than that in stage Ⅰ and Ⅱ (P <0.05). The expression of DR4, DcR1 and DcR2 in pancreatic cancer tissues had no correlation with tumor differentiation and clinical stage (P> 0.05). Conclusion ① The expression of TRAIL receptor is common in pancreatic cancer tissues, and there are differences in the expression of TRAIL receptor. TRAIL gene receptor may play an important role in the regulation of pancreatic cancer apoptosis. ②The expression of DR5 in pancreatic cancer tissues was correlated with the degree of malignancy and the degree of malignancy of pancreatic cancer. DR4 and decoy receptors DcR1 and DcR2 could not be used as indicators to judge the degree of pancreatic cancer differentiation and malignancy.