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The T cell dependence of polyclonal B lymphocyte activation (PBA) induced by one of the most important periodontopathic bacteria, Actinomyces Viscosus T14V, was investigated. Human PBL, together with Av-sup, were cocultured without or with anti-IL-2 and IL-6 monoclonal antibodies for 6 days, and cultural supernatants were harvested every 12 hours for measuring IgG, IgM, IL-2 and IL-6 activity. The results showed that (1) Av-sup was able to activate human peripheral B lymphocytes polyclonally and induce them to synthesize immunoglobulins; (2) Av-sup could also induce human PBL to produce IL-2 and IL-6 , but peaks of activity of both IL-2 and IL-6 appeared earlier than those of IgG and IgM; (3) anti-IL-2 and anti-IL-6 monoclonal antibodies could obviously depress the production of IgG and IgM, respectively, and furthermore, combined application of these two monoclonal antibodies could almost completely block the synthesis of IgG and IgM. All the data indicated that human PBA induced by Av-sup evidently de
The T cell dependence of polyclonal B lymphocyte activation (PBA) induced by one of the most important periodontopathic bacteria, Actinomyces Viscosus T14V, was investigated. Human PBL, together with Av-sup, were cocultured without or with anti-IL-2 and IL -6 monoclonal antibodies for 6 days, and cultural supernatants were harvested every 12 hours for measuring IgG, IgM, IL-2 and IL-6 activity. The results showed that (1) Av-sup was able to activate human peripheral B lymphocytes polyclonally and induce them to synthesize immunoglobulins; (2) Av-sup could also induce human PBL to produce IL-2 and IL-6, but the peaks of activity of both IL-2 and IL-6 were earlier than those of IgG and IgM; (3) anti-IL-2 and anti-IL-6 monoclonal antibodies could depress the production of IgG and IgM, respectively, and, combined, and application of these two monoclonal antibodies could almost completely block the synthesis of IgG and IgM. All the data indicated that human PBA induced by A v-sup evidently de