亚低温治疗对脑出血大鼠TLR4/NF-κB介导的神经炎症反应的影响

来源 :神经解剖学杂志 | 被引量 : 0次 | 上传用户:qingqing20090756
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的:研究亚低温治疗对脑出血后大鼠Toll样受体4(toll-like receptor 4,TLR4)/核因子-κB(nuclear factor kappa B,NF-κB)介导的神经炎症反应的影响。方法:60只成年SD大鼠随机分为假手术组(Sham组)、脑出血模型组(ICH组)和亚低温治疗组(n=20)。采用自体血注入法复制大鼠脑出血模型,并给予亚低温干预。应用Berdson评分标准对各组大鼠进行神经功能评分,放射免疫法检测肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)、白介素1β(interleukin-1beta,IL-1β)的含量;免疫组织化学法及免疫印迹法检测TLR4、NF-κB的蛋白表达情况。结果:与Sham组比,模型组大鼠神经功能评分明显增加(P<0.05),TNF-α和IL-1β的含量明显升高(P<0.05),血肿周围脑组织TLR4和NF-κB的表达显著上调(P<0.05)。与模型组相比,亚低温治疗组大鼠神经功能评分显著减少(P<0.05),TNF-α和IL-1β的含量明显降低(P<0.05),TLR4和NF-κB蛋白的表达量显著下调(P<0.05)。结论:亚低温治疗能通过调控TLR4/NF-κB表达,减轻大鼠脑出血后血肿周围脑组织的炎症反应,具有一定的神经保护作用。 Objective: To investigate the effects of mild hypothermia on neuroinflammation induced by toll-like receptor 4 (TLR4) and nuclear factor kappa B (NF-κB) in rats after intracerebral hemorrhage. Methods: Sixty adult SD rats were randomly divided into Sham group, ICH group and mild hypothermia group (n = 20). The model of intracerebral hemorrhage was reproduced by autologous blood injection, and mild hypothermia was given. The scores of neurological function were evaluated by Berdson score. The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β) were measured by radioimmunoassay. The protein expression of TLR4 and NF-κB was detected by chemical method and Western blot. Results: Compared with Sham group, the neurological scores of model group were significantly increased (P <0.05), the contents of TNF-α and IL-1β were significantly increased (P <0.05), and the expression of TLR4 and NF-κB The expression was significantly up-regulated (P <0.05). Compared with the model group, the neurological scores of the rats in the mild hypothermia group were significantly decreased (P <0.05), the levels of TNF-α and IL-1β were significantly decreased (P <0.05), and the expressions of TLR4 and NF- Down (P <0.05). Conclusion: Mild hypothermia can protect the brain tissue around the hematoma after cerebral hemorrhage by regulating the expression of TLR4 / NF-κB.
其他文献
义务教育大纲指出:义务教育的任务是为提高全民族的素质,培养有理想、有道德、有文化、有纪律的社会主义建设人才奠定基础。这就告诉我们初中物理教学的目标应该是使学生在
1997年全国物理高考试题,遵循了1997年《考试说明》中的“稳中有变”的命题原则.纵观1996、1997高考试卷,其总体结构、题型、难易题比例方面求“稳”相持平,在选择题混编、
“禽畜乐”草粉生物饲料是应用生物工程技术仿照瘤胃消化生理,将粗饲料资源——各种草杆转化为易于吸收的优化饲料,它与少量精料微量元素等配合,使三者的营养互补组成营养全
九年义务教育三年制初级中学教科书《物理》第一册,第130页.图11-4介绍了一个大气压的实验:先在瓶底铺一层沙子,把浸有酒精的棉球点燃,放入瓶内,再用剥了壳的熟鸡蛋堵住瓶口
请下载后查看,本文暂不支持在线获取查看简介。 Please download to view, this article does not support online access to view profile.
今年上半年,我国渔业生产呈现出加快发展的好势头。到5月底统计,主要省、自治区、直辖市水产品产量达508.6万吨,比上年同期增加92.9万吨,增长22.3%。除个别省市比上年同期有
长期以来,我国中学物理实验教学限于计划课时和受到各级统一考试指挥棒的影响,教师常常不敢越雷池一步,形成了教师讲解,演示实验,学生听、看实验或者是严格按照书本上既定的
在鱼场中常有烂腮、肠炎、赤皮等病发生。用茶艾合剂治疗效果很好。其方法是:取茶叶1500克,艾叶1000克,薄荷50克,大蒜杆1000克,皂角50克,加水2000毫升,共 Often in the fis
In addition to complications relating to the liver, patients with cirrhosis and portal hypertension develop extrahepatic functional disturbances of multiple org
妊娠合并梅毒未经治疗可导致自然流产、死产、非免疫性胎儿水肿、早产和新生儿先天梅毒。对所有孕妇在早孕检查时做梅毒血清学筛查并对妊娠合并梅毒患者及时规范治疗对预防先