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以四季凤仙的幼嫩茎段为外植体,MS为基本培养基,对四季凤仙的组织培养及快繁技术进行了研究。结果表明:最佳的外植体除菌方式为5%的NaClO处理5min;四季凤仙的最佳不定芽诱导培养基为:MS+6-BA 0.5mg/L+NAA 0.10mg/L+GA30.01mg/L;芽的最佳增殖培养基为:MS+6-BA1.5mg/L+NAA 0.5mg/L;最佳生根培养为:1/2MS+IAA 1.0mg/L。
In order to study the tissue culture and propagation technology of Impatiens bieti, a stem of Impatiens nigra was used as explants and MS as the basic medium. The results showed that the optimum explants were treated with 5% NaClO for 5 min, and the best adventitious bud induction medium for Impatiens nigra was MS + 6-BA 0.5 mg / L + NAA 0.10 mg / L + GA30 .01mg / L. The best bud culture medium was MS + 6-BA1.5mg / L + NAA0.5mg / L. The best rooting culture was1 / 2MS + IAA1.0mg / L.