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目的：研究致痫剂马桑内酯对谷氨酸摄取和释放功能的影响，以探讨马桑内酯致痫的可能机制。方法：放射性同位素分析技术。结果：在０．３５～３５０μｍｏｌ·Ｌ－１浓度范围内，马桑内酯使高钾（５０ｍｍｏｌ·Ｌ－１）去极化引起的脑片谷氨酸释放增加，增加率为１１．２％～３３．２％，剂量效应关系明显，３５μｍｏｌ·Ｌ－１和３５０μｍｏｌ·Ｌ－１组与对照组相比谷氨酸释放显著增加（Ｐ＜０．０１），各浓度马桑内酯对静息态谷氨酸基础释放均无明显作用。在３５０μｍｏｌ·Ｌ－１马桑内酯作用下，突触体谷氨酸摄取降低，降低率为７．９４％，与对照组差异显著（Ｐ＜０．０５），其它浓度马桑内酯作用不明显。结论：马桑内酯使谷氨酸释放增加，摄取减少，突触间隙谷氨酸聚积。
OBJECTIVE: To study the effect of epileptogenic agent (docetaxel) on glutamate uptake and release in order to explore the possible mechanism of epilepsy induced by docetaxel. Methods: Radioisotope analysis. RESULTS: In the concentration range of 0.35 ~ 350μmol·L-1, the release of glutamate from the brain of cornstalone increased with the increase of 50mmol·L-1 depolarization, the rate of increase was 11.2% ~ 33.2%, the dose-response relationship was obvious. Compared with the control group, glutamate release increased significantly in 35μmol·L-1 and 350μmol·L-1 groups (P <0.01) Basic glutamate released state had no significant effect. In the presence of 350μmol·L-1 macadamia, glutamate uptake by synaptosomes decreased by 7.94%, which was significantly different from the control group (P <0.05) Not obvious. CONCLUSIONS: Magsartolide increases glutamate release and reduces uptake of glutamate in the synaptic cleft.