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目的:研究中药复方对模拟失重大鼠承重骨对外源钙摄取的干预作用。方法:雄性Wistar大鼠随机分为3组,空白组、模型组、中药组。尾吊模拟失重,中药复方(含熟地黄、怀牛膝、黄芪、当归、牡蛎醋酸水解物等)704 g.L-1水煎剂2.5mL灌胃;适应期1周,模拟失重期3周,于模拟失重第11天一次性灌胃给予一定量41Ca示踪剂。实验结束取股骨,加速器质谱法(Accelerator Mass Spectrometry,AMS)分析骨41Ca/40Ca值,电感耦合等离子体光谱法(Inductively Coupled Plasma-AtomicEmission Spectroscopy,ICP-AES)分析骨钙总量,计算股骨41Ca沉积量、股骨41Ca沉积率。结果:空白、模型、中药3组股骨41Ca/40Ca丰度比分别为(5.03±0.55),(3.82±0.29),(5.73±0.40)×10-9,模型组较空白组显著降低(P<0.001),中药组较空白组显著升高(P<0.05);空白、模型、中药3组股骨41Ca沉积量分别为(7.39±0.90),(4.52±0.40),(7.45±0.89)×10-7mg,沉积率分别为(0.819±0.100),(0.501±0.045),(0.826±0.098)%,模型组股骨41Ca沉积量和沉积率均较空白组显著降低(P<0.001),中药组较空白组变化不显著,较模型组显著增高(P<0.001)。结论:尾吊模拟失重可造成大鼠承重骨外源钙沉积障碍,中药可较好对抗这种情况;失重骨质疏松可能存在外源钙沉积减少以外的多种机制。
OBJECTIVE: To study the effects of traditional Chinese medicine (TCM) compound on calcium intake of bone in simulated weightless rats. Methods: Male Wistar rats were randomly divided into 3 groups: blank group, model group and TCM group. Tail suspension simulation weightlessness, traditional Chinese medicine compound (including Rehmannia, Achyranthes, Astragalus, Angelica, oyster acetic acid hydrolyzate, etc.) 704 gL-1 decoction 2.5mL intragastric; adaptation period of 1 week, simulated weight loss period of 3 weeks, at On the 11th day after simulated weightlessness, a certain amount of 41Ca tracer was given intragastrically. At the end of the experiment, bone 41Ca / 40Ca value was analyzed by Accelerator Mass Spectrometry (AMS), Total Calcium and Total Calcium were measured by inductively coupled plasma-atomic emission spectroscopy (ICP-AES) Volume, femoral 41Ca deposition rate. RESULTS: The abundance ratios of 41Ca / 40Ca in blank, model and traditional Chinese medicine groups were (5.03 ± 0.55), (3.82 ± 0.29) and (5.73 ± 0.40) × 10-9, respectively, and the model group was significantly lower than the blank group (P < (P <0.05). The 41Ca deposition of blank, model and traditional Chinese medicine groups were (7.39 ± 0.90), (4.52 ± 0.40) and (7.45 ± 0.89) × 10- (0.819 ± 0.100), (0.501 ± 0.045) and (0.826 ± 0.098)%, respectively. Compared with blank group, the deposition and deposition rate of 41Ca in femur in model group were significantly lower than those in blank group (P <0.001) Group change was not significant, significantly higher than the model group (P <0.001). CONCLUSION: Simulated weightlessness in the tail hangs can cause the obstacle of exogenous calcium deposition in the bearing bones of rats, and Chinese medicine can better counteract this situation. There may exist many mechanisms besides the reduction of exogenous calcium deposition in weightless osteoporosis.