目的探讨Calcofluor White(CFW)荧光染色法对病理活检组织中真菌的检测价值。方法采用荧光增白剂CFW作为染色液,对确诊为真菌感染的21例病理活检组织中的真菌进行检测,并以经典的六胺银及PAS染色法为对照。结果荧光镜下,真菌被染成亮蓝色,与六胺银及PAS染色符合率达100%。结论 CFW荧光染色法可有效检测出组织中的真菌,值得在真菌病理活检的诊断中推广使用。 Objective To investigate the value of Calcofluor White (CFW) staining in the detection of fungi in biopsy tissues. Methods Fluorescent brightener CFW was used as staining solution to detect the fungi in 21 pathological biopsy tissues diagnosed as fungal infection. The classic hexamine silver and PAS staining methods were used as control. Results Fluoroscopy, the fungus was dyed bright blue, and hexamine silver and PAS staining in line with the rate of 100%. Conclusion CFW fluorescence staining can effectively detect the fungi in tissues and is worth popularizing in the diagnosis of fungal pathology biopsy.