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作者采用微量技术从干血纸片抽提 DNA,从约50μl 全血中可获得0.5μg DNA.将抽提得的 DNA 和 Y-特异 DNA 探针进行点杂交和 Southern 杂交,能快速鉴定人类性别.这种微量DNA 抽提技术有助于把重组 DNA 技术应用于边远地区人群的遗传病诊断和研究.
The authors used micro-extraction of DNA from dried blood disks to obtain 0.5 μg of DNA from approximately 50 μl of whole blood.The spotted DNA and Y-specific DNA probes were subjected to dot blot and Southern blot to rapidly identify human gender This trace DNA extraction technique can help to apply recombinant DNA technology in the diagnosis and research of genetic diseases in remote areas.