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1 材料和方法1.1 材料胃粘膜组织标本72例来自1998-10/1999-10我院内镜胃粘膜活检标本.每例患者在胃粘膜相邻部位取6块粘膜组织.2块立即放入经处理的试管中后置入液氮中,然后送-80℃低温冰箱保存待检.4块作病理检查.胃粘膜标本72例经病理证实胃腺癌(GC)33例,胃粘膜重度异型增生8例,轻度异型增生5例,胃粘膜肠上皮化生(ICM)7例,慢性浅表性胃炎(CSG)19例.1.2 方法1.2.1 组织提取物制备取冰冻活检组织30mg～50mg 置入消毒过的玻璃匀浆器中,于冰水浴中匀浆,加入 200μL冷裂解液,于0℃冰水中孵育30min 后20 000 r/min 高速离心20min,
1 Materials and Methods 1.1 Materials Gastric mucosa specimens of 72 cases from 1998-10 / 1999-10 in our hospital endoscopic gastric biopsy specimens of each patient in the gastric mucosa adjacent to take 6 mucosal tissue .2 immediately into the menstrual Treated tubes were placed in liquid nitrogen, and then sent to -80 ℃ low temperature refrigerator to be tested .4 for pathological examination.Seventy-two cases of gastric mucosa were confirmed by pathology of gastric adenocarcinoma (GC) 33 cases, severe dysplasia of gastric mucosa 8 5 cases of mild dysplasia, 7 cases of intestinal mucosal intestinal metaplasia (ICM), 19 cases of chronic superficial gastritis (CSG) .1.2 Methods 1.2.1 Preparation of tissue extracts Frozen biopsy 30mg ~ 50mg Sterilized glass homogenizer, homogenized in an ice-water bath, 200 μL of cold lysate was added and centrifuged at 20 000 r / min for 20 min at 0 ° C in ice water for 30 min.