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为克隆人 B7.2 c DNA,构建 B7.2真核表达质粒 ,并在哺乳动物细胞中表达。分离正常人淋巴结淋巴细胞 ,经 LPS诱导后 ,以 RT- PCR,克隆 B7.2 c DNA;构建真核表达质粒 p BCMGSNeo- B7.2 ,转染哺乳细胞 ,进行表达。结果成功克隆了 B7.2c DNA,并经测序证实 :所构建的 B7.2抗原真核表达质粒可在哺乳动物细胞中高效表达。表明经 LPS诱导的人淋巴细胞可表达 B7.2 m RNA,所建立的 p BCMGSNeo-B7.2哺乳动物真核表达质粒 ,可供进一步研究 B7.2结构和功能。
To clone human B7.2 c DNA, a B7.2 eukaryotic expression plasmid was constructed and expressed in mammalian cells. The normal human lymph node lymphocytes were isolated and induced by LPS. The B7.2 c DNA was cloned by RT-PCR. The eukaryotic expression plasmid p BCMGSNeo-B7.2 was constructed and transfected into mammalian cells for expression. Results The B7.2c DNA was successfully cloned and confirmed by sequencing. The eukaryotic expression plasmid of B7.2 antigen was highly expressed in mammalian cells. It is indicated that human lymphocytes induced by LPS can express B7.2 m RNA and the eukaryotic expression plasmid of p BCMGSNeo-B7.2 mammalian can be used to further study the structure and function of B7.2.