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目的:建立测定血浆和脑组织中文拉法辛的方法。方法:血浆和脑组织样品均用液-液萃取法,正己烷∶异戊醇(99∶1)萃取。色谱柱Agilent Zorbax Eclipse×DB-C8(4.6 mm×150 mm,5μm),流动相以乙腈∶磷酸钾缓冲液(25∶75);流速1.4 ml/min;室温;进样量20μl;检测波长229 nm。结果:在0.25~32.00μg/ml范围内,血浆中文拉法辛浓度与峰面积的线性关系良好(r=0.9999);在0.05~6.40μg/ml,脑组织中文拉法辛浓度与峰面积也呈良好的线性关系(r=0.9994)。血浆回收率为89.4%,血浆中文拉法辛浓度在0.5,4.0,32.0μg/ml的日内变异系数(SD)分别为3.7%~5.5%,1.6%~5.2%和4.2%~5.7%,日间SD分别为6.5%,5.0%和2.0%,最低检测浓度为0.15μg/ml。脑匀浆回收率是93.3%,脑匀浆文拉法辛浓度在0.5,4.0,32.0μg/ml的日内SD分别为2.2%~3.4%,1.3%~6.0%和0.6%~3.4%,日间SD分别为4.1%,3.4%和5.6%,最低检测线为0.25μg/ml。结论:建立了文拉法辛在血浆和脑组织中的高效液相色谱分离测定方法。该方法准确、重复性好、选择性强,适用于大鼠体内药代动力学研究和脑组织中药物浓度的测定。
Objective: To establish a method for the determination of venlafaxine in plasma and brain tissue. Methods: Plasma and brain tissue samples were extracted by liquid-liquid extraction, n-hexane: isoamyl alcohol (99: 1). Column Agilent Zorbax Eclipse × DB-C8 (4.6 mm × 150 mm, 5 μm) with mobile phase of acetonitrile: potassium phosphate buffer (25:75), flow rate of 1.4 ml / min, room temperature, injection volume of 20 μl, detection wavelength of 229 nm. Results: There was a good linear relationship between the concentration of venlafaxine and peak area in the range of 0.25 ~ 32.00μg / ml (r = 0.9999) Showed a good linear relationship (r = 0.9994). The plasma recovery was 89.4%, the intra-day coefficient of variation (SD) of plasma desvenlafaxine concentrations were 3.7% -5.5%, 1.6% -5.2% and 4.2% -5.7% at 0.5, 4.0 and 3.0μg / The SDs were 6.5%, 5.0% and 2.0% respectively, with the lowest detection concentration of 0.15μg / ml. The brain homogenate recovery was 93.3%. The SD of cerebral homogenate venlafaxine was 2.2% ~ 3.4%, 1.3% ~ 6.0% and 0.6% ~ 3.4% at the days of 0.5, 4.0 and 3.0μg / The SDs were 4.1%, 3.4% and 5.6% respectively, with the lowest detection line of 0.25μg / ml. Conclusion: Venlafaxine was established for the determination of venlafaxine in plasma and brain tissue by high performance liquid chromatography. The method is accurate, reproducible and selective. It is suitable for the study of pharmacokinetics in rats and the determination of drug concentration in brain tissue.