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对长白山地区木贼山柰素含量和反射光谱参数的关系进行了研究,探索了判别木贼药材质量优劣的快捷方法。根据反射光谱曲线(Unispec-Sc光谱分析仪)导出的若干参数,对木贼样品进行聚类分析,采用HPLC法分别测定木贼山柰素的含量,分析了反射光谱参数与山柰素含量的相关性。结果表明:蓝边面积(SDb)、绿峰反射率(Rg)等参数与山柰素含量呈显著负相关(r分别为-0.991,-0.982),530～570nm波段的反射率与山柰素含量亦呈显著的负相关(︱r︱>0.95)。通过比较SDb、Rg,可简捷地判断长白山地区木贼质量的优劣。增强530～570nm波段的入射光线,可刺激山柰素含量的增加,但需要大田实验进一步验证。
The relationship between the content of cantharidin and the reflection spectrum parameters of Equisetum sinica was studied in Changbai Mountain, and the shortcut method to judge the quality of Equisetum camellia was explored. According to some parameters derived from the curve of the reflection spectrum (Unispec-Sc spectrometer), the samples of H. bidentata were clustered and analyzed by HPLC. The content of puerarin was determined by HPLC. The correlation between the reflectance spectra parameters and the content of kaempferol Correlation. The results showed that there was a significant negative correlation between the parameters SDb and Rg, and the content of kaempferol (r = -0.991, -0.982, respectively). The reflectance of 530 ~ 570nm band was positively correlated with kaempferol There was also a significant negative correlation (︱ r︱> 0.95). By comparing SDb, Rg, Changbai Mountain area can be concisely judge the quality of Equisetum pros and cons. Enhancing the incident light in the 530-570 nm band can stimulate the increase of the content of the Shan Ku Su, but it needs the field experiment to further verify.